Friday, March 16, 2018

Youtube daily report Mar 16 2018

Hey everyone, welcome back to my channel. In today's video I will be sharing with you how to make your very own vision board for 2018.

For more infomation >> DIY: How To Make Your Own Vision Board - Duration: 6:20.

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Mel B bares boobs in plunging bralette with intimate bedroom shot - Duration: 2:49.

Mel B bares boobs in plunging bralette with intimate bedroom shot

It has been a tough year for Scary Spice after publicly going through the courts and divorce with her ex-husband Stephen Belafonte, after she claimed he had a relationship with the nanny and suffered from domestic violence in the marriage.

Sadly the case has not ended there and Belafonte is fighting for visitation rights to see Mel B and Eddie Murphy's 10-year-old daughter Angel, saying he raised her throughout her life as his own.

As filming for America's Got Talent begins, Mel seems unfazed by the whole affair and has certainly shown Stephen what he is missing after posting a body-baring snap on Instagram.

  Gem water is a process of restructuring the water, charging it with negative ions and energy by putting specific crystals into your drinking water.

It is claimed to be a good energy booster and stress reliever.

Although fans didn't seem interested in her new water system, as they liked the post over 70,000 times and filled her with compliments.

  A second gushed: "Damn, you've never looked finer.

" While a third devoted admirer gushed: "So sexy… Hot morning Miss Mel B.

" You would never believe, the former Spice Girl was a 42-year-old mother of three, with such an age-defying physique.

  Confidently posing up a storm by her mum's side, Phoenix Chi Gulzar opted for a daring naked illusion dress that was covered in sequins and slashed up the middle, completing the look with delicate black sandals.

Phoenix seems to be following in her mother's footsteps after growing a 37K fanbase of her own on Instagram, and branching out with a sucessful YouTube channel. .

For more infomation >> Mel B bares boobs in plunging bralette with intimate bedroom shot - Duration: 2:49.

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El superyate que quita el sueño a Arantxa S. Vicario tras su divorcio de Josep Santacana - Duration: 7:02.

For more infomation >> El superyate que quita el sueño a Arantxa S. Vicario tras su divorcio de Josep Santacana - Duration: 7:02.

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2018 USSV RHINO GX DRIVING THE FORD F 450 BASED OFFENSIVE DEFENDER - Duration: 5:12.

If the best defense is indeed a good offense, then this rebodied Ford F-450 must be the

most offensive—and hence best defending—mode of road transport available, at least to those

whose personal defense budget can swing a quarter-million bucks.

Oh, and although this U.S. Specialty Vehicles Rhino GX looks bulletproof, making it literally

so (to B6-level gunfire resistance) adds considerable cost.

What do you get for the price of two Hellfire air-to-ground missiles?

Quite a bit more than meets the eye—which is an entirely new SUV body where a regular-cab

and 8-foot dualie pickup box was when the Ford F-450 Super Duty pickup left the Kentucky

Truck Plant in Louisville.

The original truck's frame, firewall, A- and B-pillars are retained and heavily modified.

The rest of the new bodywork is constructed of hand-worked steel, except for the hood,

front fenders, and all four wheel flares, which are composite pieces.

Please note the hand-holds at the roof and matching foot indentations in the lower doors

that allow your retinue of expendable bodyguards to hang onto the outside of the vehicle and

provide cover fire with their trigger hands as you whisk through unfriendly territory.

Sharp eyes will note the last-gen Super Duty headlights have also been retained.

Yes, production orders through this summer are being filled on 2016-vintage truck chassis,

after which the tooling will all be revised to accommodate the 2017 and newer F-450.

The front suspension is largely stock, augmented with Bilstein adjustable shocks.

The rear suspension is completely redesigned, however.

The leaf springs are ditched in favor of a CLASS (Compressible Liquid Active Suspension

System) from LiquidSpring (suppliers to the ambulance, bus, and RV industries).

The axle is located by upper and lower radius rods on both sides with a lateral link above

the axle.

Hydraulic spring/damper units provide load leveling, three ride-height settings (the

front is not adjustable), and variable damping.

The forged aluminum wheels are minor miracles of engineering as well, because they are interchangeable

front to rear, simply by turning them around.

This is no easy task, given that the donor truck places the rear wheel mounting surface

well outboard of the fronts because of the F-450's standard dual-rear-wheel setup.

But by machining and painting the inside and outside, and by merely swapping the center

caps, a deeply offset rear wheel can be inverted to fit on the shallow-offset front axle.Inside,

the stock front seats are dismantled, fancier foam is installed, and they're re-swathed

in a more luxurious grade of hand-sewn leather that now covers virtually everything you can

see or reach—except the headliner, which is Alcantara.

The second and third rows are specially constructed for USSV.

The Sync system is ditched in favor of an Alpine touchscreen infotainment system that

also provides control of the extensive auxiliary LED lighting that can produce near daylight

all around the truck.

An Executive configuration features a partition housing a big-screen TV, with more luxurious

thrones set farther back in lieu of third-row seating.

So what does it feel like to drive this gigantic black Rhino?

Intimidating—in the active, "I'm the intimidator" sense of the word.

One enjoys a sense of respect from fellow motorists that is utterly absent from the

helm of a Ford EcoSport or Chevrolet Trax.

Forward visibility is good, but the view out the rear is iffy (a great high-mounted camera

affords a commanding view when reversing, however).

When accelerating, the Power Stroke diesel seems to pretty well shrug off the 2,500 or

so pounds USSV loads the Super Duty underpinnings with.

Steering is big-rig lifeless but accurate enough (to paraphrase the saying, close enough

for horseshoes, hand grenades, and nuclear warfare).

Clearly this rig isn't for everyone—especially not every American.

Of the 300 or so Rhino GXs USSV has moved so far, only 20 of them are stomping around

on U.S. soil.

The vast majority are sold in China, where it's called the G. Patton.

Yes, after that iconic American champion of a good offense, Old Blood and Guts George

S. Patton.

But stay tuned—USSV is about to introduce its Rhino XT, a two-door rebody of a Jeep

Wrangler Unlimited.

Perhaps in China, this one will be named after a slightly less bellicose general—maybe

the D. Eisenhower.

For more infomation >> 2018 USSV RHINO GX DRIVING THE FORD F 450 BASED OFFENSIVE DEFENDER - Duration: 5:12.

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LEGO® Ninjago® Season 8 ...

For more infomation >> LEGO® Ninjago® Season 8 ...

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Jaguar XE 2.0D 180 R-SPORT / WINTER / BUSINESS - Duration: 1:01.

For more infomation >> Jaguar XE 2.0D 180 R-SPORT / WINTER / BUSINESS - Duration: 1:01.

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Jaguar XE 2.0D AUT PRO EDITION PURE / PREM. BUSINESS - Duration: 0:54.

For more infomation >> Jaguar XE 2.0D AUT PRO EDITION PURE / PREM. BUSINESS - Duration: 0:54.

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Mercedes-Benz E-Klasse E 220 d Limousine Automaat Lease Edition - Duration: 0:57.

For more infomation >> Mercedes-Benz E-Klasse E 220 d Limousine Automaat Lease Edition - Duration: 0:57.

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Mercedes-Benz E-Klasse E 220 d Limousine Automaat Lease Edition - Duration: 0:43.

For more infomation >> Mercedes-Benz E-Klasse E 220 d Limousine Automaat Lease Edition - Duration: 0:43.

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Mercedes-Benz E-Klasse E 220 d Limousine Automaat Avantgarde | Spiegelpakket | COMAND - Duration: 0:59.

For more infomation >> Mercedes-Benz E-Klasse E 220 d Limousine Automaat Avantgarde | Spiegelpakket | COMAND - Duration: 0:59.

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Mercedes-Benz GLA-Klasse GLA 180 d Automaat | AMG Line | Panorama schuifdak - Duration: 0:57.

For more infomation >> Mercedes-Benz GLA-Klasse GLA 180 d Automaat | AMG Line | Panorama schuifdak - Duration: 0:57.

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Toyota HiAce 2.5 D-4D KWB - Duration: 1:00.

For more infomation >> Toyota HiAce 2.5 D-4D KWB - Duration: 1:00.

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CS 1.6 OYNUYORUM :d - Duration: 2:48.

For more infomation >> CS 1.6 OYNUYORUM :d - Duration: 2:48.

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Kia cee'd Plus Pack met Navi, Cruise, Climate en LMV - Duration: 1:01.

For more infomation >> Kia cee'd Plus Pack met Navi, Cruise, Climate en LMV - Duration: 1:01.

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Tempesta D'Amore anticipazioni 19-25 marzo: un ricatto e un matrimonio annullato - Duration: 4:35.

For more infomation >> Tempesta D'Amore anticipazioni 19-25 marzo: un ricatto e un matrimonio annullato - Duration: 4:35.

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18 incroyables bienfaits du chlorure de magnésium | Santé 24.7 - Duration: 8:49.

For more infomation >> 18 incroyables bienfaits du chlorure de magnésium | Santé 24.7 - Duration: 8:49.

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La famille Hally­day devant la justice : pourquoi l'affaire ne pour­rait pas être réglée à l'amiable - Duration: 1:58.

For more infomation >> La famille Hally­day devant la justice : pourquoi l'affaire ne pour­rait pas être réglée à l'amiable - Duration: 1:58.

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I'm Still Not Buying Limited Run Games Anymore (but I Still Love the Company!) - Duration: 5:32.

Welcome to Burning Books.

I'm Caleb, and I want to help you love video games even more.

And if you are a fan of Limited Run Games, I'm about to help you love those games even

more.

As you now have a better chance of owning those limited release games, because I'm not buying

them anymore.

I'm one less buyer to compete with.

I made a video about Limited Run Games a month ago.

I'd like to make a better one.

That's what this is (hopefully it's better).

I'm going to talk about why I love what Limited Run Games does and why I'm not buying as many

games from them as I used to.

Sounds contradictory, I know...and it probably is.

I'm still trying to figure out the logic in my own head.

Maybe you can help me out in the comments below.

What am I thinking?

Limited Run Games is a company that distributes limited quantities of previously digital only

video games for the PS4, PS Vita, and recently for the Nintendo Switch.

This simple business model runs in direct opposition to the rest of the digital-forward

gaming industry.

Limited Run Games' value proposition then is to keep the physical medium alive.

This naturally taps into the collector market, and by making the games limited, they make

that collector market ravenous.

Each release sells out within minutes.

And I, since October 2016 have been one of those ravenous collectors, counting down the

seconds prior to each release, waiting for the clock to strike 9:00am Central Standard

Time, ready to act quick to secure every PS4 release.

I later found some good deals on pre-October releases, and before I knew it I had accumulated

an unbroken series, starting with the first release, Saturday Morning RPG, released in

January 2016.

This meant, I was hooked.

I was in deep.

Maintaining the series became more important than vetting the quality of the games.

Not that the games are bad, but the fact that I didn't even question the quality is what's

important here.

And this absence of questioning is what seeded my decision to cut back on purchases.

My backlog grew.

It grew and grew and grew.

Not only did 3-4 new Limited Run Games releases land on my shelf every month, but all those

other exciting releases came too: Breath of the Wild, Super Mario Odyssey, Nier: Automata to name just a few of the amazing 2017 games.

And every one of those new release purchase--though very exciting for me--was tinged with regret.

Or, perhaps, sadness is a better word.

A sadness that those backlogged Limited Run Games were getting pushed further and further

down the list.

As of today, I've played only 37 of the 68 Limited Run Games PS4 Releases.

And one day, I realized I couldn't do it anymore.

There are just way too many games I want to play, and I don't have time to play games

I'm not really excited to play.

I understand it's not uncommon for video game collectors to own games they've never played

and never intend to play.

But that's not me.

And once I realized this, the decision to cut back on buying video games was easy.

I had a clear flowchart: is this a game I will play?

Buy it.

Is this a game I won't play?

Don't buy it.

And to be fair, I didn't just say no to Limited Run Games releases.

I'm cutting back on all games.

Limited Run Games just happened to be my biggest problem.

Cutting them off meant cutting off a huge expense because keep in mind I'm referring

to those games as a continuum purchase, not as individual purchases.

I was buying to maintain the series.

Without a maintained series, I can now be more discriminating with the games I decide

to purchase from them in the future.

And it's important that I emphasize just how much I love Limited Run Games as a company.

I buy into their why, their reason for being.

I talk more about the company's why in my "Limited Run Games Releases Saturday Morning

RPG on the Switch" video, link in the description below.

I bought this shirt after deciding that I was going to cut back on buying the games

because I still wanted to support the company somehow.

Limited Run Games is helping to keep physical media alive.

This is important.

Many people don't realize that buying digital isn't ownership.

You are not legally allowed to transfer ownership of that file.

When you die, your digital games die with you.

I learned this from VGCollectaholic, a smart guy and my co-host at the Masters of Unlocking

podcast.

Check us out.

Half of us is smart.

Lastly, it's important to note that me not purchasing Limited Run Games releases anymore won't hurt

their bottom line.

They sell out of every release every time.

In fact, if you are a collector of Limited Run Games, you should be happy.

I'm one less person buying the game you want.

My original video from a month ago is still available should you want to watch it, link

in the description below.

I stand by my opinion, so I'm not unpublishing the video.

However, per various comments on the video I felt I could do a better job articulating

the nuances of my decision to stop buying so many Limited Run Games releases.

I hope I've done that here.

If anything, think of it this way.

I will now enjoy the games I do play even more, meaning I'm a much more enthusiastic

video game citizen.

I'm a better member of the community, this community we all love so much.

Please like, subscribe, and click the Bell icon to make sure you don't miss future videos.

If you are still watching this video, you obviously like it, right?

So, it only makes watch more and subscribe.

For more infomation >> I'm Still Not Buying Limited Run Games Anymore (but I Still Love the Company!) - Duration: 5:32.

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Dancing Bear - Tita & Tedi - Duration: 2:06.

Hy Bacon's! Today isn't an ordinary day!

As you know I'm still in Germany

So I had to thing about Something quickly made and who might be interesting.

Did you already see a dancing bear?

Me Neither...

Thanks For watching this video!

If you liked, give a thumbs Up!

All the music's links will be down below.

You will find there to, my twitter and my Facebook.

If you want to see all my other videos, subscribe there!

Thanks to Boris you help me in this video.

For more infomation >> Dancing Bear - Tita & Tedi - Duration: 2:06.

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*FACE REVEAL* The Origin the Kraken - Duration: 10:18.

Welcome to another episode of Origins of Monstrosity live edition oh yeah.

Today we'll be talking about the Kraken . The Kraken is a sea monster that originates of

the coast of Norway and Greenland.

People suspect the legend originates from sailors and civilians seeing a giant squid

40 to 50 feet long.

The word Kraken stems from the Norwegian word "krake" which means an unhealthy animal or

something twisted.

In the late 15th century version of the Icelandic saga Orvar-Oddr- or- it's Norwegian- er- Icelandic,

Icelandic, I'm not going to get it the first time.

You got it bud.

Okay, there is a story of a journey bound for Baffin Island which takes the protagonists

through the Greenland Sea where they meet the two sea monsters.

First is the Hafgufa.

Hafgufa, Hafgufa - the first is the Hafgufa which means sea mist and the second is the

Lyngbackr heatherback.

I'm sorry for my speech impediment!

You don't have a speech impediment.

Prove it.

The Hafgufa is a reference to the Kraken.

A passage reads: "The Hafgufa is the hugest monster in the sea."The narrator - hugest

isn't a word.

It was in the book Ben!

Carry on.

The narrator of the Norwegian work Konugs Skuggja - you tried your best.

It's Norwegian Ben!

I'm not from Norway!

That's why I said you tried your best.

Calm down.

Okay - says there must be only one Hafgufa in existence as it is only seen in the same

area of the Greenland Sea and that it is incapable of reproduction as its numbers never seem

to grow.

Wow that's really a depressing life, you can't even have sex what is life without sex , at

least for an animal.

Anyway, a passage from the book reads: "Most often in our tongue we call it the Hafgufa

or Kraken.

He has appeared more like land than a fish."

He's saying it looks more like an island than a fish because the original book is really

dumb.

Yeah,he's saying it looks like an island.

That;s what i said.

Yeah, well you said it wrong.

Continue.

Carl Linnaeus classified the Kraken as a Cephalopod and gave it the scientific name Microcosmus

marinus _ hold up hold up hold up.

You're saying that they gave a scientific name to a fake animal that doesn't exist.

Kidding folks, it exists.

Yeah, Carl Linnaeus did. and if you let me finish my sentence though - he gave it the

scientific name Microcosmus marinus in the first edition of his book Systema Naturae.

He excluded the Kraken from any further editions of the book.

Good, can you say that first word in the scientific name please?

Microcosmus.

Why is "micro" in the name of a really really big creature?

Because - I don't think this guy has all his marbles I'm not gonna lie.

In a scientific book he put "micro" in the name of a huge creature in a scientific book

and not only a creature that does not exist.

In another book he wrote he describes the Kraken as a unique creature and writes:"It

is said to inhabit the seas of Norway but I have not seen this animal."

What a surprise and coincidental.

The Kraken was also heavily described by the bishop of Bergen, Bergen, Bergen, Erik Pontoppidan.

No, Pontoppidan.

That's what I said, listen to the audio.

He made many speculations including that sometimes people mistake an island for a Kraken.

Yes, that would make sense, islands look very animal-like, I've seen them.

He also wrote - but no but like if an animal is stationary for a long period of time, like

logs and stuff, you can grow like - Have you ever seen Avatar the Last Airbender?

There is a really big turtle in that that actually is an island.

Yeah see.

He also wrote that: "It is said that if the creature's arms were to lay hold of the largest

man-of-war they would pull it down to the bottom."

And then Swedish author Jacob Wallenberg wrote about the Kraken in his 1781 work "My Son

of the Gallery" - he has a son that is Kraken?

No - gosh that kid is deformed!

"My S0n of the Gallery."

He just writes about - it's just cruelty to call your son a Kraken.

Calling your son a Kraken, oh my goodness.

Do you want me to read the whole passage?

No thank you.

That's enough.

I'm going to read the whole passage.

No its okay, you're alright.

"Kraken, also called the Crab-fish, which is not that huge, for heads and tails counted,

he is no larger than our Öland is wide...

He stays at the sea floor, constantly surrounded by innumerable small fishes, who serve as

his food and are fed by him in return: for his meal, (if I remember correctly what E.

Pontoppidan writes,) lasts no longer than three months, and another three are then needed

to digest it.

His excrements nurture in the following an army of lesser fish, and for this reason,

fishermen plumb after his resting place ... Gradually, Kraken ascends to the surface, and when he

is at ten to twelve fathoms, the boats had better move out of his vicinity, as he will

shortly thereafter burst up, like a floating island, spurting water from his dreadful nostrils

and making ring waves around him, which can reach many miles.

Could one doubt that this is the Leviathan of Job?"

Yes they could, because it doesn't exist.

The Leviathan of Job does.

Sure.

French.

Oh, okay, I don't know how to say that word because - French malacologist.

French (mumbles) Let me read this, let me read this.

That was my French accent.

French malacologist Pierre Denys de Monfort, see there you go.

My French accent was better.

Wrote about two types of giant octopuses, one was the Kraken octopus and then that it

had been described by Norwegian sailors, American whalers - that rhymes.

That's a new song I just came up with.

Norwegian sailors American whalers.

Anyway, and seen by people like - Pliny the Elder, which is kinda incredible considering

he the one who saw Pompeii and stuff.

Well, he's also on weed continue.

No Ben, he's like super - have you never heard of Pliny the Elder?

I haven't but no that he's saying that he's seen Krakens - How?

Hes - I'm pretty sure he's - Monfort also claimed that ten British warships including

the French Ville de Paris had been attacked and sunk by giant octopuses after the ships

went missing in 1782.

The British knew from a survivor of the event that the ships had actually sunk during a

hurricane off the coast of Newfoundland, so they made Monfort to look like a fool.

It's not the fact that he believed in giant octopuses, but the fact that he thought a

boat- that a - that he got wrong why a boat went down, that's what made him a fool?

Because he said "hey the Kraken did this" but anyway, continue.

That's not one word.

It was an accident.

You forgot a space there ma'am.

Yeah I did.

The Kraken legend continues to this day in television, movies, books, video games, and

even roller coasters.

I like roller coasters, do you like - yeah so do I, roller coasters are great.

Uh, I actually'd like to read the full Alfred Tennyson - that's too bad.

In 1830 Alfred Tennyson published the sonnet called the Kraken.

A passage from it reads: Below the thunders of the upper deep;

Far far beneath in the abysmal sea, His ancient, dreamless, uninvaded sleep

The Kraken sleepeth: faintest sunlights flee About his shadowy sides; above him swell

Huge sponges of millennial growth and height; And far away into the sickly light,

From many a wondrous grot and secret cell Unnumber'd and enormous polypi

Winnow with giant arms the slumbering green.

There hath he lain for ages, and will lie Battening upon huge seaworms in his sleep,

Until the latter fire shall heat the deep; Then once by man and angels to be seen,

In roaring he shall rise and on the surface die.

That's fun.

Yeah.

In Chapter 59 of Herman Melville's Moby Dick - a great book by the way I read it in 4th

grade.

I thought it had too much Moby too little Dick.

Pequod encounters chief - Pequod, Pequod encounters what chief-mate Starbuck -Starbuck! here I'll

read the thing.

Oh my gosh chief-mate Starbuck - yeah - Get me a latte right now.

describes as "The great live squid, which, they say, few whale-ships ever beheld, and

returned to their ports to tell of it" it was in chapter 59.

Here - I already said that.

Okay, whatever, read from here, Ismael.

Who's Ishmael?

The narrator.

There's a narrator in a book?

Not the narrator One of the most famous lines in a books like ever.

It's the first line in the book - he's like "I am Ishmael."

He's like I, singular.

The most famous line is a guy saying I am Ishmael?

It's - well -It's iconic.

I guess you guys just need to like catch up.

It's iconic.

Like movies you have "Luke I am your father."

I could just read it.

Diehard, its just like - I'll just read it, I'll just read it.

Like uh, what's another good one.

Um, um.

Or you just can just keep talking.

Terminator, Terminator has some great ones.

That'snot a book.

I'm just saying how movies are better than books.

did you know that the line "Here's Johnny" was improvised.

He improvised it.

That's great right?

Ishmael adds: "There seems some ground to imagine that the great Kraken of Bishop Pontoppidan

may ultimately resolve itself into Squid."

He ends the chapter by saying: "By some naturalists who have vaguely heard rumors of the mysterious

creature, here spoken of, it is included among the class of cuttle-fish, to which, indeed,

in certain external respects it would seem to belong, but only as the Anak of the tribe."

So you're saying the Kraken is a cuttle-fish.

Where can I buy said cuttle-fish to cuddle with said cuttle-fish.

Cuttle like c-u-t-t-l-e fish.

Yes.

Not cuddle, like c-u-d-d-l-e.

Um, I can't spell.

In the television show gang of Thrones - Game of Thrones - excuse me, The Greyjoy's sigil

is a Kraken.

You know that, you've - you're in the middle of watching the show.

I am not, this is fake news, that show is for women.

No he's not he on season two episode two.

Was that sexist?

I don't know.

Thanks for watching!

If you enjoyed the video, like and subscribe for more.

Or don't.

Or don't.

Comment below if you have any questions or if you have anything you'd like a video on

comment that below as well.

Or do you like this new style or do you think our faces are dumb.

I think - or that.

I think our faces are great.

Hey, you know what, our faces are cool, faces are nice.

Faces are always cool.

Okay I'm going to stop now, bye.

For more infomation >> *FACE REVEAL* The Origin the Kraken - Duration: 10:18.

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Automated map sharpening with phenix.autosharpen - Duration: 6:05.

Today we are going to learn about automatic map sharpening using auto sharpen in the Phenix

GUI.

Have a look at this cryo-EM map.

We are going to blur and sharpen this map by adjusting the resolution-dependent weighting

of the map.

As the map is sharpened the clarity initially improves but eventually it becomes worse as

the connectivity is lost.

The numbers in the upper right corner indicate the final B-iso of the map, a measure of its

sharpness where lower means sharper.

Notice that the map is most interpretable when the B-iso value is about 20 in this case

Phenix autosharpen is going to automatically choose the sharpening of the map that leads

to the highest level of detail while maintaining the connectivity of the map.

This combination is called "adjusted surface area" because the clarity is highest when

the surface area of the contours in the map is highest.

In most cases maximizing adjusted surface area leads to an optimally sharpened map.

The required inputs for map sharpening are a map file and the high-resolution limit of

the map.

The map file can be any standard format such as an mrc map or a ccp4-style map or MTZ file.

The map can come from cryo-EM or from X-ray crystallography.

The resolution limit is used to reduce the contribution of high-resolution noise to the

map.

For a cryo-EM map you can use the resolution limit you have identified using half-dataset

Fourier Shell Correlations.

For an X-ray map you can use the resolution of your data.

Here is the oversharpened rotavirus map that we would like to auto-sharpen.

Let's open the Phenix GUI and optimize the sharpening of this map.

In the main Phenix GUI, click "new project" and then "set up tutorial data".

Then under "please select a dataset" go down to the Cryo-EM datasets and pick the "autosharpen

map" tutorial.

Choose a suitable place to put this data and hit OK

Now there will be a new rotavirus auto sharpening project ready for you to use.

To run auto sharpen, click on Maps and then scroll down and click on "Autosharpen map"

This will set up a new autosharpen run.

Let's put in a title

We can choose our map file by clicking on browse which will take us to the directory

where we copied the tutorial data.

Select "rotavirus over sharpened map and click Open to load the file.

Let's set the resolution to 2.6 A.

Now we're ready to go.

Hit Run and the autosharpen GUI will try to automatically figure out the optimal sharpening

of this map.

As the run starts, a new autosharpen tab will appear and the log file is displayed in the

Run Status window.

Here the log file is telling us about the map we supplied.

Notice the grid and unit cell and make sure these agree with what you expect.

Also notice the origin and extent of the map.

The origin of cryo-EM maps is not necessarily at (0,0,0) and often is instead at the center

of the map.

Auto-sharpen will write out the sharpened map with the same origin as the input map.

Here autosharpen is identifying the value of the final sharpening parameter B-iso that

maximizes the adjusted surface area (SA), which is the combination of map clarity and

connectivity.

The best value of B-iso is 100, with an adjusted surface area value of 3.9

This corresponds to a sharpening B applied of minus 86, that is, the map is blurred compared

to the starting map.

At the end of the run, a summary is presented.

It lists the final sharpening B applied of -86.

It lists the file containing the sharpened map as well.

If we click on the "Open in coot" button, the sharpened map is displayed.

The sharpened map is quite nice.

It looks very little like the original over-sharpened map.

However note that these two maps have exactly the same information in them.

It is just that the weighting of Fourier terms at high resolution is different in the two

maps.

The over sharpened map has large Fourier coefficients at high resolution and the optimally sharpened

map has lower values.

In this case it was necessary to blur the map to optimize it.

In other cases it is necessary to sharpen it.

Auto-sharpen can also be used to optimally sharpen a map to best fit a model.

This can sometimes give just a little bit better sharpening the without a model, but

it also has just a little bit of bias towards the model.

In the autosharpen window you just enter the model.

You can also use autosharpen to sharpen locally, with a different value in different parts

of the map.

To do this, go to all parameters in the autosharpen window, then click on map modification, and

turn on local sharpening.

Good luck with automatically sharpening your map!

If you have questions or feedback you are welcome to contact us at help at phenix online

dot org or tutorials at phenix online dot org

For more infomation >> Automated map sharpening with phenix.autosharpen - Duration: 6:05.

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OVER AND OVER AGAIN by NATHAN SYKES in ASL (American Sign Language) - Duration: 4:01.

From the way you smile to the way you look

You capture me unlike no other

From the first hello, yeah, that's all it took

And suddenly we had each other

And I won't leave you

Always be true

One plus one, two for life

Over and over again

So, don't ever think I need more

I've got the one to live for

No one else will do, and I'm telling you

Just put your heart in my hand

Promise it won't get broken

We'll never forget this moment

Yeah, we'll stay brand-new 'cause I'll love you

Over and over again

Over and over again

From the heat of night to the break of day

I'll keep you safe and hold you forever

And the sparks will fly, they will never fade

'Cause every day gets better and better

And I won't leave you

Always be true

One plus one, two for life

Over and over again

So, don't ever think I need more

I've got the one to live for

No one else will do, yeah, I'm telling you

Just put your heart in my hands

I promise it won't get broken

We'll never forget this moment

Yeah, we'll stay brand-new 'cause I'll love you

Over and over again

Over and over again

Girl, when I'm with you I lose track of time

When I'm without you you're stuck on my mind

Be all you need till the day that I die

I'll love you

Over and over again

So, don't ever think I need more

I've got the one to live for

No-one else will do, yeah, I'm telling you

Just put your heart in my hands

Promise it won't get broken

We'll never forget this moment

Yeah, we'll stay brand-new 'cause I love you

Over and over again

Yeah, over and over again

Over and over again

For more infomation >> OVER AND OVER AGAIN by NATHAN SYKES in ASL (American Sign Language) - Duration: 4:01.

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Laeti­cia, direc­trice artis­tique de Johnny Hally­day ? « Elle n'a pas les compé­tences » - Duration: 2:07.

For more infomation >> Laeti­cia, direc­trice artis­tique de Johnny Hally­day ? « Elle n'a pas les compé­tences » - Duration: 2:07.

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D'Urso: Eva non sarà più nei suoi programmi, Per quale motivo è stata presa questa decisione? - Duration: 3:29.

For more infomation >> D'Urso: Eva non sarà più nei suoi programmi, Per quale motivo è stata presa questa decisione? - Duration: 3:29.

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【星野花】3回目の質問コーナー / 3rd Q&A 【7年記念/7th Anniversary】 - Duration: 7:02.

Hello everyone! Hana here~

Since my channel has reached it's seventh year

Today I'll be holding my 3rd Q&A!!

Thank you all for writting questions in order for me to be able to make this video~

So let's answer the questions~

Do you like pandas?

Personally it's not my favorite animal, but I like them, they are cute~

I already talked about this in another Q&As but

I never had any japanese lessons

I simply bought a book and a dictionary and I started studying from there

but lately I study by watching japanese tv programs and by listening to japanese music and trying to translate the meaning

Which is the reason why you started to like Hello!Project?

Watching "Kimagure Princess"'s music video and thinking that they were awesome

made me search about Morning Musume and then I found Hello!Project

they make music with different styles

Their performance level is good

They are all so funny and cute and I love the feeling of "family" you have in Hello!Project

Those were the reasons

Have you ever went to a Hello!Project live?

Sadly I never went to one..

but once day I want to

I really want to watch their performance live

Which is your favorite song from Hello!Project?

It changes with time but "Shabondama" might be my favorite

I really like that song

The performance of "Shabondama" really leaves a big impression

And then I also like "Memory Seishun no Hikari" a lot

Do you have interest on old Hello!Project? For example: Melon Kinenbi or Taiyou to Ciscomoon

Yes, I have interest

Melon Kinenbi's "Akai Freesia"

"I'll believe in it Red Freesia"

I really like that song and also

"Reflecting on the champagne's glass My lover's gentle lips"

I like "Champagne no Koi" a lot too

From Taiyou and Ciscomoon I really like that "Gatamekira" is a great song

No matter how many times I've listened to it I'm always surprised by their amazing vocals

I really listen to that song a lot

Also, from old groups I love Coconuts Musume a lot.

Funakki is the member I like the most in Country Girl's, who is your favorite member in Country Girls?

Momochi was my favorite member

but since she graduated it became Yanamin

What do you like in Maachan?

I find Maachan a very special girl. I like her since her audition.

She's cute, funny and her performance level is always improving.

Also I love the fact that she's always so energetic

Airi's solo debut song was upload to YouTube and other websites. Have you listened to it yet? I find it good but I want to listen to your impressions.

I listened!! I am really looking forward to her solo debut (the songs sound really good!!)

If she release's a MV I'll certainly do a reaction.

Have you watched the Olympics? Japan's curling female team managed to get the bronze medal

and somehow they are all from my city. Now the city is on a welcome festival mode!!

Everyone in the city must be so happy!! Congratulations!!!

As for the Olympics, I didn't really watch it...

Winter Olympics are not that popular in Portugal

We only had 3 athletes representing Portugal (I checked it and it seems that there were only 2 XD I'm sorry for the mistake!)

But I saw the Ski Jumping and Figure Skating events

In Figure Skating I was rooting for Evgenia Medvedeva and Yuzuru Hanyu

Also I was very surprised by the positive with the japanese athlete that was in his 40's in the Ski Jumping event

What are the little things in life that make you the happiest?

Listening to the rain while you are so confortable at home

Eating chocolate

Being with my family, laughing with my friends

Doing a task that was difficult for you but you managed to do it!

If you could be anyone for a day, who would you be?

To be honest I wouldn't like to be another person

because when I was little, I actually wanted that, I never liked myself and I always wanted to be like someone else

But nowadays it's not really the case, I don't really want to be another person. I am starting to like myself so

you know, rather than being another person for a day, I would like to time travel for a day

I would love to see my city, but 100 years ago

There are some buildings that are now so old and I would love to see on how they were back then

How did people dress up back then

How was society back then

I think it would be a good experience

You are going to laugh

but the thing that scares me the most is going to the dentist

you know, I really hate going to the dentist,

I get so nervous! I really hate it xD

Why are you so cute

I have a very low self-esteem

So thank you for helping my self esteem with those comments

Really, thank you

Last question!

On programs like Hello!Station there is a cooking corner. In Hana's hometown do you make japanese food? I would like to see you challenge a simple japanese plate.

It wasn't in my hometown but in 2017's January I ate sushi for the first time

It was very good

I would like to try making japanese food

But which plate/recipe would it be better?

Please write in the comments simple japanese food recipes.

I want to challenge it and if I do it, I'll make a iudeo.

That's it

Thank you all for the questions!!

When I saw all the questions I really wanted to thank you from the bottom of my heart. I was very happy.

Fromnow on I am planning on keeping doing my best, so please support me.

It has been Hana, let's meet in a next video!

For more infomation >> 【星野花】3回目の質問コーナー / 3rd Q&A 【7年記念/7th Anniversary】 - Duration: 7:02.

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2018 USSV RHINO GX DRIVING THE FORD F 450 BASED OFFENSIVE DEFENDER - Duration: 5:12.

If the best defense is indeed a good offense, then this rebodied Ford F-450 must be the

most offensive—and hence best defending—mode of road transport available, at least to those

whose personal defense budget can swing a quarter-million bucks.

Oh, and although this U.S. Specialty Vehicles Rhino GX looks bulletproof, making it literally

so (to B6-level gunfire resistance) adds considerable cost.

What do you get for the price of two Hellfire air-to-ground missiles?

Quite a bit more than meets the eye—which is an entirely new SUV body where a regular-cab

and 8-foot dualie pickup box was when the Ford F-450 Super Duty pickup left the Kentucky

Truck Plant in Louisville.

The original truck's frame, firewall, A- and B-pillars are retained and heavily modified.

The rest of the new bodywork is constructed of hand-worked steel, except for the hood,

front fenders, and all four wheel flares, which are composite pieces.

Please note the hand-holds at the roof and matching foot indentations in the lower doors

that allow your retinue of expendable bodyguards to hang onto the outside of the vehicle and

provide cover fire with their trigger hands as you whisk through unfriendly territory.

Sharp eyes will note the last-gen Super Duty headlights have also been retained.

Yes, production orders through this summer are being filled on 2016-vintage truck chassis,

after which the tooling will all be revised to accommodate the 2017 and newer F-450.

The front suspension is largely stock, augmented with Bilstein adjustable shocks.

The rear suspension is completely redesigned, however.

The leaf springs are ditched in favor of a CLASS (Compressible Liquid Active Suspension

System) from LiquidSpring (suppliers to the ambulance, bus, and RV industries).

The axle is located by upper and lower radius rods on both sides with a lateral link above

the axle.

Hydraulic spring/damper units provide load leveling, three ride-height settings (the

front is not adjustable), and variable damping.

The forged aluminum wheels are minor miracles of engineering as well, because they are interchangeable

front to rear, simply by turning them around.

This is no easy task, given that the donor truck places the rear wheel mounting surface

well outboard of the fronts because of the F-450's standard dual-rear-wheel setup.

But by machining and painting the inside and outside, and by merely swapping the center

caps, a deeply offset rear wheel can be inverted to fit on the shallow-offset front axle.Inside,

the stock front seats are dismantled, fancier foam is installed, and they're re-swathed

in a more luxurious grade of hand-sewn leather that now covers virtually everything you can

see or reach—except the headliner, which is Alcantara.

The second and third rows are specially constructed for USSV.

The Sync system is ditched in favor of an Alpine touchscreen infotainment system that

also provides control of the extensive auxiliary LED lighting that can produce near daylight

all around the truck.

An Executive configuration features a partition housing a big-screen TV, with more luxurious

thrones set farther back in lieu of third-row seating.

So what does it feel like to drive this gigantic black Rhino?

Intimidating—in the active, "I'm the intimidator" sense of the word.

One enjoys a sense of respect from fellow motorists that is utterly absent from the

helm of a Ford EcoSport or Chevrolet Trax.

Forward visibility is good, but the view out the rear is iffy (a great high-mounted camera

affords a commanding view when reversing, however).

When accelerating, the Power Stroke diesel seems to pretty well shrug off the 2,500 or

so pounds USSV loads the Super Duty underpinnings with.

Steering is big-rig lifeless but accurate enough (to paraphrase the saying, close enough

for horseshoes, hand grenades, and nuclear warfare).

Clearly this rig isn't for everyone—especially not every American.

Of the 300 or so Rhino GXs USSV has moved so far, only 20 of them are stomping around

on U.S. soil.

The vast majority are sold in China, where it's called the G. Patton.

Yes, after that iconic American champion of a good offense, Old Blood and Guts George

S. Patton.

But stay tuned—USSV is about to introduce its Rhino XT, a two-door rebody of a Jeep

Wrangler Unlimited.

Perhaps in China, this one will be named after a slightly less bellicose general—maybe

the D. Eisenhower.

For more infomation >> 2018 USSV RHINO GX DRIVING THE FORD F 450 BASED OFFENSIVE DEFENDER - Duration: 5:12.

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[KOR SUB] REACTION! BTS Mic Drop Fancam 방탄소년단 - Duration: 8:31.

Hello, everybody, my name is Maggie, and I like to react to K-pop videos on the Internet.

Welcome to my channel, or welcome back to my channel, or welcome back to my channel,

whatever your case may be.

It is so good to have you here, thank you so much.

All right!

Today we are going to be taking a look at a Mic Drop fancam, and I'm totally excited,

and completely terrified.

This, uh, I got this footage from a content creator named Mera, I will link the channel

up in here in the cards.

Before we dive in, every single week I run a Twitter poll.

You decide what I upload and react to on Mondays.

My Twitter handle is @ReactionaryMag, so please go and participate in the Twitter polls.

They have been so close.

I'm so, I'm so excited, but they have been really close.

Other than that, I don't know.

I'm, uh, I do.

Okay.

I have my support system.

I've got Scotty the bear.

We're just going to move him a little bit closer to me.

I also have the emergency kit, the first aid.

So we'll see if I need either of these.

Or both.

Which is a high possibility, because Mic Drop.

All right.

Let us do the thing.

They all look so mad.

Seriously, one of my favorite dances.

Such attitude.

I've actually never seen it live though, I don't think. No, not like this.

Let's do this, J-Hope.

Oh no.

You're going to screw me up! Oh no! Damn you, Tae.

Wasn't expecting it.

Whoo! How cool does RM look?

I can't with that guy.

Love him so much.

I have a really funny Mic Drop story that I'll tell you at the end.

Oh!

Oh my god.

Okay.

This is a lot.This is a lot. We're going straight to the hard stuff.

This is lit. This is a lot.

And I'm kind of okay that it doesn't have, you know, fire lighting up at the back and stuff like that.

I just want to see them.

This is really, really, really, really, really spicy though.

Oh, it's my Mafia! I haven't seen you guys for a minute!

I'm sorry, excuse me, what?

What? What is going on?

What, I, oh no. I'm not ready.

I am not mentally, spiritually, emotionally prepared for this.

I got to get right with my Lord and Savior, which is music.

Are you angels or demons?

How cool does RM look? Oh my god. Can I be your buddy?

Oh, god. Whoops. Whoops!

Was Suga late on that?

These two. These two. These two. Uh-uh. Uh-uh. Uh-uh. Mm-mm. Nope.

Then you add that, mm-mm.

That's when done happens.

JIn, you're getting it too! Oh my god. I, I'm approaching done. Rapidly.

What?

I am in so much trouble right now. No, my god, what is this?

J-Hope and dancing were made to go together.

This is, okay, I, I, I, I, what?

What a spectacular performance so far.

Yeah they are. Screw them. That's right.

I am about as good at dancing as, you know, that. I can do that.

Oh my god. Okay.

You know it's bad when I have to move the ice.

Okay.

Symmetry.

Oh, my Mafia. Oh god, we still have 38 seconds? Uh oh, what's going to happen?

Wrong!

No!

I am destroyed!

Who needs anything to destroy them that's worse than that? Oh my god! Okay.

That was really, really, really, really everything.

How much do I love you? Oh! Where's the mic drop?

We missed the mic drop. That's okay.

I've seen it before.

I've only seen that on like, variety talk shows.

You know, when they were on Jimmy Kimmel and stuff like that.

I have never seen a full blown situation which caused me to have a full blown situation.

Oh my god, my heart is in my throat.

Okay. Here's my funny Mic Drop story.

My sister and I, um, have very different tastes in music when it comes to some things, but

we both love EDM.

And we were going to a massive EDM concert and I was doing her makeup, and she had it

on shuffle, and Mic Drop Steve Aoki mix came on, and I stood in that bathroom and did my

version of the dance, and by my version, I mean it was probably just the worst thing

you've ever seen but I don't care.

So my sister laughed at me for a solid five minutes, and it was actually really fun.

Oh my god.

I need to see these guys live.

I feel like the energy that they give to the crowd and that the crowd gives to them is

just something special and something that creates a life of its own.

If we don't see these types of concerts, and interact with them, as fans like myself, you

know, in America, in the middle of America, who is probably going to have to fly to a

different part of the world or country to see a concert, this is the only way I can

access this type of content, you know?

Is to look at this, then to react to it, then to provide commentary and share it with you

guys, and I hope that you have fun when I do that.

This one wrecked me. Yeah.

This was fantastic. So I hope that you liked this.

If you did, please subscribe to my channel, I would love to have you.

And when you do that, please press the notification bell, again, so you are notified when I upload.

And if you give this video a big like, I will find some more fancams of songs.

I, I feel like I haven't covered a song in a little bit.

So let me know what you would like to see, I will put some more videos that I think you

might like in the cards, and until the next time we are hanging out together on this YouTube

channel, I hope you have a really great day.

Take care. Bye bye.

For more infomation >> [KOR SUB] REACTION! BTS Mic Drop Fancam 방탄소년단 - Duration: 8:31.

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DIY: How To Make Your Own Vision Board - Duration: 6:20.

Hey everyone, welcome back to my channel. In today's video I will be sharing with you how to make your very own vision board for 2018.

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