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The last few days
We've been on an expedition in the rain forests of Ecuador and our goal was to take a portable lab into the field
sequence the DNA of an organism and identify it to species in real time
so this was part of a National Geographic grant and
With a team of herpetologists here in Ecuador our goal was to use a new portable sequencer
This is a device that was made by Oxford nanopore technologies. It's this really cool portable gene sequencer
We use some other equipment like the miniPCR and
Overall we were able to take the bare bone elements of a laboratory and take it out into a very remote
rainforest in Ecuador. And so why would we want to do this?
essentially we are in a very biodiverse region of the planet and we went to a
Region called Canande which is in the Choco rainforest of Ecuador and this region is highly diverse
there are lots of species that are known some that are threatened some that are undescribed and
So the problem is that this place is being deforested very rapidly
So there's a real time sensitivity to trying to understand the organisms that live there
There is a very strong need to actually preserve this biodiversity here
Actually bringing the sequencer to the field
can save time. So we can instead of like going out in the field collecting the samples
bringing it back to a lab having them send off to a different country for sequencing this can take weeks and months and
In our case it took less than 24 hours
It was a really exciting turnaround we went out with our portable lab
And we found animals that night like vipers, geckos, frogs. All of these amazing diverse organisms
And so we were able to take a small amount of blood
extract DNA use PCR amplification for certain genes that are used in identifying species
And then we sequenced it right than in there so in less than 24 hours we had received the information
The DNA information that we could identify them to species
accurately and
A cool thing is that you can even do the bioinformatics in the field the only thing you need is a laptop
So it's very very easy very fast
And you get actual sequence you can even run a phylogeny or blast it against the database to see what species it is
in the rainforest and this has actually lots of
Implications not just for conservation or for teaching people about the biodiversity in certain areas
But also for labs in countries like Ecuador where all the sequencing technologies is not readily available
so if we can do it in the field anyone can do it in the lab here, and so this technology really works well for
Scientist here to apply it in the labs
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