Developing a treatment for cancer has been one of the biggest missions in the
history of science and medicine. Research has been conducted on multiple methods
believed to be the end-all treatment for this malicious disease, but none have
yielded the results that were expected or hoped for. Gaining a better
understanding of the physiological functions involved in these speculated
treatments will save scientists time energy and resources by expediting the
process of affirming or disproving the efficacy of possible cancer treatments.
Younger USA, LLC holds the tool necessary for revealing these physiological functions
that will be the key to finding the ultimate cancer treatment. Non-invasive
Micro-testing technology, or NMT, provides the ability to detect and measure the
real time flux of selective ionic and/or molecular activities on situ
in live samples ranging from single cells of five micrometers to tissue ad
whole organs, as well as observe activities of tumor microenvironments.
In "Recombinant horseradish peroxidase variants for targeted cancer treatment"
published in the Journal of Cancer Medicine in 2016, the enzyme horseradish
peroxidase, HRP, combined with indole acetic acid, IAA, was researched as a powerful
targeted anti-cancer agent. This was the first study describing the successful
use of recombinantly produced HRP for targeted cancer treatment, and this
discovery might lead to an increased use of the powerful isoenzyme HRP C1A in
future cancer research.
This study focused on single tumor cells. In order to advance further it is also
necessary to observe the activity of IAA within the tumor microenvironment. By
limiting the span of observation to single cells, other factors such as
signaling molecules immune cells and blood vessels that surround the tumor
are ignored. These aspects need to be considered when observing a tumor as
evidence shows they have substantial influence on the behavior and biology of
the cancer. Observing the effects of IAA/HRP on the tissue level, the tumor as a
whole, and the interaction between the tumor cells and their surrounding
environment would provide significant progress in this research as it would
reveal deeper functions involved in this phenomenon.
The types of treatments
studied in this paper are gene and antibody directed enzyme pro drug
therapy, which allow the selective release of a cytotoxic agent from a non-toxic
prodrug, in this case IAA, at the site of a tumor. With the NMT, the activities and
functions of IAA can be studied as the interaction between the treatment and
tumor occurs in real-time. NMT can provide crucial data, including which
part of the tissue is responsible for the uptake of IAA and where the
conversion occurred, as well as the concentration and location of endogenous
IAA in and around the tumor. NMT could also be used to observe the
effects of blocking ion transportation sites, which would provide proof of how
IAA/HRP effects cancer cells and tumors. Thanks for watching, see you next time!
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