Tuesday, December 26, 2017

Youtube daily report Dec 26 2017

R&B singer G.Soul leaves message for fans on military enlistment

Soul leaves message for fans on military enlistment.   R&B singer G.Soul has officially enlisted for his mandatory military service.

  On December 26, a rep revealed the singer started his enlistment in Kangwon Province, and after 4 weeks of training, hell be moving for his official military service.G.Soul said through his agency, Im not sure if Ill be a big help, but I feel very proud that Im able to defend my country.

Ill work hard in my army life and return with a more healthy body and mind. Ill work hard doing music once again when Im out.After 16 years with JYP Entertainment, G.Soul recently signed with H1GHER MUSIC.Good luck to G.Soul!.

For more infomation >> R&B singer G.Soul leaves message for fans on military enlistment(News) - Duration: 1:05.

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Gemma e Giorgio sono tornati insieme? La verità | K.N.B.T - Duration: 3:33.

For more infomation >> Gemma e Giorgio sono tornati insieme? La verità | K.N.B.T - Duration: 3:33.

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Anticipazioni Uomini e donne 26 dicembre: va in onda oppure no? | M.C.G.S - Duration: 3:36.

For more infomation >> Anticipazioni Uomini e donne 26 dicembre: va in onda oppure no? | M.C.G.S - Duration: 3:36.

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Quartz-Seq2 (Just after Single Cell Collection --- cryopreservation of single-cell plate) - Duration: 7:48.

Immediately after the cell collection

The 384 well PCR plate was temporarily sealed with LightCycler 480 Sealing Foil (Roche)

The sealed 384-well PCR plate was centrifuged at 10,000 g and 4ºC for 1 min using TOMY MX307, equipped with a Rack-in-Rotor and centrifugation rack PCR96-02

These steps were very important to collect the droplet with a single cell in lysis buffer in an efficient manner.

By altering the volume of lysis buffer from 0.4 µL (as described in the original Quartz-Seq paper) to 1 µL (Quartz-Seq2), bubbling of lysis buffer was not required before single-cell sorting and the lysis buffer could easily be handled.

We then peeled open the temporary seal

We eliminated static electricity of the plate by an ionizer

We re-sealed it with Agilent PlateLoc Thermal Microplate Sealer (165ºC 1 sec) or Biorad PX1 PCR Plate Sealer (180ºC 3 sec) at 4ºC

We agitated the plate at 2,600 rpm and 4ºC for 1 min using ThermoMixer C (Eppendorf)

We centrifuged the plate again (10,000 g and 4ºC for 1 min)

We put the plate in a plastic bag

The resulting 384-well plate was then immediately cryopreserved at −80ºC and maintained under such conditions until subsequent reverse transcription for cell barcoding

After cryopreservation, we performed subsequent whole-transcript amplification using the cryopreserved 384-well PCR plate within a few months

For more infomation >> Quartz-Seq2 (Just after Single Cell Collection --- cryopreservation of single-cell plate) - Duration: 7:48.

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Uomini e Donne oggi, crisi forte tra Sonia e Emanuele: Natale da separati - Duration: 3:15.

For more infomation >> Uomini e Donne oggi, crisi forte tra Sonia e Emanuele: Natale da separati - Duration: 3:15.

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Google Pixel 2 Review & First Look | Google Phone Beyond Your Imagination - Duration: 2:05.

Google Pixel Phone

For more infomation >> Google Pixel 2 Review & First Look | Google Phone Beyond Your Imagination - Duration: 2:05.

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기아차, 첨단 안전기술 대거 적용한 K7 출시..그랜저에 도전장 - Duration: 4:47.

For more infomation >> 기아차, 첨단 안전기술 대거 적용한 K7 출시..그랜저에 도전장 - Duration: 4:47.

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MODERN COMBAT 5 #1 AWAKENING RAIDERS + RESCUE - Duration: 12:30.

For more infomation >> MODERN COMBAT 5 #1 AWAKENING RAIDERS + RESCUE - Duration: 12:30.

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奇跡体験!アンビリバボー! 南の島国で尊敬される男!国を変えた日本人・壮絶な人生! - Duration: 44:59.

For more infomation >> 奇跡体験!アンビリバボー! 南の島国で尊敬される男!国を変えた日本人・壮絶な人生! - Duration: 44:59.

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GF VIP: Cecilia Rodriguez e Ignazio Moser: Natale a casa di Belen | Wind Zuiden - Duration: 3:02.

For more infomation >> GF VIP: Cecilia Rodriguez e Ignazio Moser: Natale a casa di Belen | Wind Zuiden - Duration: 3:02.

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If You EAT GINGER Every Day 👉❤ Look What Happens 👌💯 - Duration: 4:31.

If You EAT GINGER Every Day 👉❤ Look What Happens 👌💯

For more infomation >> If You EAT GINGER Every Day 👉❤ Look What Happens 👌💯 - Duration: 4:31.

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GF VIP: Anticipazioni L'isola dei famosi 2018 cast: ecco chi sono i naufraghi | Wind Zuiden - Duration: 3:42.

For more infomation >> GF VIP: Anticipazioni L'isola dei famosi 2018 cast: ecco chi sono i naufraghi | Wind Zuiden - Duration: 3:42.

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Albano e Romina power: Loredana Lecciso e donna Jolanda hanno fatto pace? | Wind Zuiden - Duration: 3:30.

For more infomation >> Albano e Romina power: Loredana Lecciso e donna Jolanda hanno fatto pace? | Wind Zuiden - Duration: 3:30.

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Aksi Istimewa Anis Merah Teler Sampe Doyong Istimewa - Duration: 1:17.

For more infomation >> Aksi Istimewa Anis Merah Teler Sampe Doyong Istimewa - Duration: 1:17.

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Kelakuan Anis Merah Teler Klasik Tapi Sangat Istimewa - Duration: 1:21.

For more infomation >> Kelakuan Anis Merah Teler Klasik Tapi Sangat Istimewa - Duration: 1:21.

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Гонки с тележкой по магазину. Racing with the cart on the store. - Duration: 2:13.

For more infomation >> Гонки с тележкой по магазину. Racing with the cart on the store. - Duration: 2:13.

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한끼줍쇼 김남주 집에 방탄소년단 정국 진 김승우 삼성동 80억 집 띵동!|K-News - Duration: 6:19.

For more infomation >> 한끼줍쇼 김남주 집에 방탄소년단 정국 진 김승우 삼성동 80억 집 띵동!|K-News - Duration: 6:19.

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Don't Drink and Drive

For more infomation >> Don't Drink and Drive

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Downpours in Oman. What happened on our Planet. What happened in the world. - Duration: 1:51.

At Oman, south-east of the Arabian Peninsula, collapsed

torrential rains, due to which It has suffered several

human.

Rescuers had to evacuate people from the car caught

in turbulent flow.

Dozens of people were locked in their

homes.

Torrential rains from 16 December 2017, and is projected

Professionals can continue whole week.

The world already has Tens of millions of people,

forced to leave their homes because of natural disasters.

Each year, this figure is growing.

In the near future climate Refugees will be billions!

Accommodation on the huge devastated, flooded

elements infected (due man-made disasters)

territories will be impossible.

Meanwhile, the majority of people are still blithely

pinning hope, responsibility for their lives and expectations

assistance and any action on those who run away first

at the slightest threat.

All people should think: If now, in a relatively

peacetime, priests and politicians the world's elite are concerned

only software of its own well-being and salvation

their families, what will In the near future - in

time of global cataclysms?

Assume that the problem of survival all mankind touches

you personally, your children and grandchildren, extremely hindsight,

this is equivalent to that starve yourself, your children

and grandchildren to perish ...

For more infomation >> Downpours in Oman. What happened on our Planet. What happened in the world. - Duration: 1:51.

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Google Pixel 2 Review & First Look | Google Phone Beyond Your Imagination - Duration: 2:05.

Google Pixel Phone

For more infomation >> Google Pixel 2 Review & First Look | Google Phone Beyond Your Imagination - Duration: 2:05.

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Candlelight 2017 [CC] - Duration: 18:32.

We're at a Epcot, for candlelight tonight! We're gonna go look for Chip and Dale

things and eat dinner at Chef De France later. They have the new rosegold long-sleeve

shirts. I still like the purple one better. Hillary: I like my rosegold one. Cute.

Christmas Tree!

It's like so warm out today, I don't know why, it's like 85 I'm gonna keep saying

that, I brought my fan with me, all right.

She bought the Cronut. I've never had

one of these, I heard they're really really good. Hillary: Wanna try it?

Yes! I'm not gonna eat---well I might, but you should try a piece.

There's one chip and dale, I have to back up because you can't see it.

That is their ornament, it's a deer

The menu for Chef De France. I'm probably gonna get the mac and cheese, uhhh.

French onion soup and then chocolate cake.

Are we gonna see the baes? Yeah. hehe, YEAH.

[Silver Bells]

Look at all this gingerbread.

And a lot of pixie dust!

If I zoom in will it be easier to find them....it's not.

The ornaments maybe? oh oh I see one up there at the very very top. I see it. Hillary: If that's how small they're gonna be we're never gonna find it.

oh there's one on top of the roof.

[Three Caballeros Instrumental]

Hillary: It's Coco!

I wonder if they put him in here.

Dolan.

That's me, nonononono.

HIllary: Look they're on the flags up there, I never noticed that.

[Three Caballeros] as preformed by Jose, Panchito, and Donald. A Masterpiece.

I think Donald needs a haircut.

Hey. We said that at the exact same time. I'm watching doki-doki literature Club and we're waiting for Mostly Kosher

What does that mean? Mostly Kosher. Does that mean one of them is not Jewish?

We had Chef De France, it was really good, I'll

insert some pictures right now and then we're gonna go get in line for

candlelight after this because it shows at 5:15 and then our Candlelight show is

at 6:45 and then I think we're gonna stay for illuminations...so yeah, we're all still

here in our food comas so good times ahead.

CJ: Applying makeup.

And staring at her phone... wow this really focuses like I turn the camera

and it... whoa. Hillary: It does what its supposed to. CJ: This is cool.

I like it, I like this camera, it's cool.

Hillary: Why did you give CJ your camera? Kayla: I didn't she took it. Hillary: This is why we don't give CJ cameras.

[Lots of sick Jewish songs mixed with pop culture songs, good stuff]

[Jump for Joy]

Neil Patrick Harris.

The Candlelight Processional I'm Neil, I am so honored to be here with you all

at Epcot which I I'm pretty sure stands for every parent can order tequila.

This is my tenth year as a narrator and I just, we had a lovely little celebration just

backstage and the crew gathered together and they gave me spaceship earth. I thought that was so thoughtful.

We've...our family gets to come here and I love the tradition of it all, I love this show

the holidays are amazing and it's fun to do things every year and also it's fun

to watch firsts. Our kids are seven and they have a lot of firsts now because

they're the right height to ride rides. Harper...Harper road rock and roller

coaster for the first time this year and I wept. Gideon road the Tower of

Terror for the first time.... and he wept.

So it's memories that we'll all remember for the rest of our lives.

But I'm glad you've chosen to spend a little bit of your very fast pass day

of running around to sit still for a few minutes and listen to these wonderful Carol's

performed by these exquisite performers.

City of Galilee named Nazareth to a virgin

who was betrothed to a man whose name was Joseph of the house of David and the

Virgin's name was Mary and he came to her and said greetings o

favored one the Lord is with you. But she was greatly troubled at the

saying and tried to discern what sort of greeting this might be

and the angel said to her do not be afraid Mary for you have found favor

with God and behold you will conceive in your womb and bear a son and you shall

call his name Jesus

[o come let us adore him]

[O Holy Night]

Glory to God in the highest and on earth, peace, goodwill towards men.

Came to Joseph and said father Joseph the organ is broken and we will have no

music for the Christmas Eve service...heh. Well later in the day as Joseph made his

rounds he visited a home where a baby had just been born and he thought about

the birth of the baby Jesus and he was so inspired that he wrote a poem and

went back to the church and found Franz. He said if we can't have the old organ

maybe we can have a new song and you can play the guitar and Franz said father I

only know three chords. heh. heh. Then three chords is all you'll need

so Franz hummed and strummed and soon he had composed the most beautiful and

beloved of all the Christmas carols.

[Silent Night]

We're back home and we're watching some more doki-doki.

And it's the last night of Hanukkah how pretty. Hillary: This is seriously so much light,

The miracle of Hanukkah is... damn we could light our whole apartment with this thing right now.

Lookin' pretty good... uh-oh my battery's about to die perfect timing, say bye. BYE!

you

For more infomation >> Candlelight 2017 [CC] - Duration: 18:32.

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[LIEN VOSTFR] EXO - Universe - Duration: 0:11.

For more infomation >> [LIEN VOSTFR] EXO - Universe - Duration: 0:11.

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L'Ayurvéda est très Zéro Déchet ! | Natalia Apvril - Duration: 3:35.

For more infomation >> L'Ayurvéda est très Zéro Déchet ! | Natalia Apvril - Duration: 3:35.

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Waterfalls of Queureuillh and of Rossignolet in Mont Dore in Puy de Dôme - France - Duration: 4:06.

Hello and welcome. Today I am in the Massif du Sancy

in the Puy de Dôme

in the town called Mont Dore

And I am going to see the Waterfall of Queureuilh and its sister, the Waterfall of Rossignolet

Waterfalls of France presents

Waterfall of Queureuilh and Waterfall of Rossignolet - Puy de Dôme

Waterfall of Queureuilh

For the Waterfall of Queureuilh

We cannot realize its size, because there is no element that can show it

Go in your compositions

in ordre to add a scale, so people can realize of its 20 meters height

I am leaving now the Waterfall of Queureuilh

to go and see the Waterfall of Rossignolet

The path is where I came,

this here, is the path I will take

Waterfall of Rossignolet

So why is ti called this way (Rossignol = nightingale) ?

People say

that we can here

the sound of a nightingale

down the river

So I am going to record the sound of the water

the river after the waterfall, like the legend says

and we will compare with the sound of a nightingale

Let's do it now

here si the scientist test

Here is the sound of the water

next to the Waterfall as the legend says

And here is a sound of nightingale

you can find here in Auvergne

LOL

You've discovered the Waterfall of Queureuilh

and the Waterfall of Rossignolet

In Mont Dore - Puy de Dôme

So if you liked this video

please leave a thumb's up

and leave me a comment

if you know this waterfall

Time to say bye now and I ll see you with another watefall

Look with your eyes and not with you mobile devices

This a generation thing

This a generation thing

Ok, so yes look with your eyes

but think about taking your camera with you

because it's gorgeous :)

and don't forget with you tablet

to watch the video of Waterfalls of France ;)

For more infomation >> Waterfalls of Queureuillh and of Rossignolet in Mont Dore in Puy de Dôme - France - Duration: 4:06.

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What is Best Time To Do Spel...

For more infomation >> What is Best Time To Do Spel...

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K-State ready to take on UCLA at Cactus Bowl Tuesday night - Duration: 2:03.

For more infomation >> K-State ready to take on UCLA at Cactus Bowl Tuesday night - Duration: 2:03.

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한끼줍쇼 김남주 집에 방탄소년단 정국 진 김승우 삼성동 80억 집 띵동!|K-News - Duration: 6:19.

For more infomation >> 한끼줍쇼 김남주 집에 방탄소년단 정국 진 김승우 삼성동 80억 집 띵동!|K-News - Duration: 6:19.

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Uomini e Donne gossip Sara Affi Fella: Nicola ha una nuova fidanzata - Duration: 2:46.

For more infomation >> Uomini e Donne gossip Sara Affi Fella: Nicola ha una nuova fidanzata - Duration: 2:46.

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R&B singer G.Soul leaves message for fans on military enlistment(News) - Duration: 1:05.

R&B singer G.Soul leaves message for fans on military enlistment

Soul leaves message for fans on military enlistment.   R&B singer G.Soul has officially enlisted for his mandatory military service.

  On December 26, a rep revealed the singer started his enlistment in Kangwon Province, and after 4 weeks of training, hell be moving for his official military service.G.Soul said through his agency, Im not sure if Ill be a big help, but I feel very proud that Im able to defend my country.

Ill work hard in my army life and return with a more healthy body and mind. Ill work hard doing music once again when Im out.After 16 years with JYP Entertainment, G.Soul recently signed with H1GHER MUSIC.Good luck to G.Soul!.

For more infomation >> R&B singer G.Soul leaves message for fans on military enlistment(News) - Duration: 1:05.

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Fake 2PM - Taecyeon Birthday - Duration: 0:36.

Hyung!

It's been so long!

Wow! you're so awesome!

Minjun: Tacyeon~

I love you Taecyeon

I missed you

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